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Hits 101 – 120 of 501

101
Estratégias de relativização nas variedades lusófonas
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102
Retextualização da escrita por alunos com surdez usuários da LIBRAS: em foco a mediação do professor
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103
Análise do curso de extensão em Libras, na modalidade a distância, para formação continuada de professores no contexto educacional inclusivo
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104
Alunos com surdez e o processo de inclusão: uma análise dos discursos sobre língua de sinais
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105
Alunos com surdez e o processo de inclusão: uma análise de discursos sobre sobre Língua de Sinais
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106
CO-FORMING REAL SPACE BLENDS IN TACTILE SIGNED LANGUAGE DIALOGUES
In: DeafBlind Culture and Community (2015)
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107
Your mum! Teenagers' swearing by mother in English, Spanish and Norwegian
In: International journal of corpus linguistics. - Amsterdam [u.a.] : Benjamins 19 (2014) 1, 29-59
OLC Linguistik
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108
Coding causal–noncausal verb alternations: A form–frequency correspondence explanation – CORRIGENDUM
In: Journal of linguistics. - London [u.a.] : Cambridge Univ. Press 50 (2014) 3, 779
OLC Linguistik
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109
A test case for an optimality-theoretic approach to left-edge metathesis
In: Proceedings of the forty-ninth (49.) annual meeting of the Chicago Linguistic Society (2014), S. 1-16
Leibniz-Zentrum Allgemeine Sprachwissenschaft
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110
COSMID: A Web-based Tool for Identifying and Validating CRISPR/Cas Off-target Sites
Cradick, Thomas J; Qiu, Peng; Lee, Ciaran M; Fine, Eli J; Bao, Gang. - : Nature Publishing Group, 2014
Abstract: Precise genome editing using engineered nucleases can significantly facilitate biological studies and disease treatment. In particular, clustered regularly interspaced short palindromic repeats (CRISPR) with CRISPR-associated (Cas) proteins are a potentially powerful tool for modifying a genome by targeted cleavage of DNA sequences complementary to designed guide strand RNAs. Although CRISPR/Cas systems can have on-target cleavage rates close to the transfection rates, they may also have relatively high off-target cleavage at similar genomic sites that contain one or more base pair mismatches, and insertions or deletions relative to the guide strand. We have developed a bioinformatics-based tool, COSMID (CRISPR Off-target Sites with Mismatches, Insertions, and Deletions) that searches genomes for potential off-target sites (http://crispr.bme.gatech.edu). Based on the user-supplied guide strand and input parameters, COSMID identifies potential off-target sites with the specified number of mismatched bases and insertions or deletions when compared with the guide strand. For each site, amplification primers optimal for the chosen application are also given as output. This ranked-list of potential off-target sites assists the choice and evaluation of intended target sites, thus helping the design of CRISPR/Cas systems with minimal off-target effects, as well as the identification and quantification of CRISPR/Cas induced off-target cleavage in cells.
Keyword: Original Article
URL: http://www.ncbi.nlm.nih.gov/pubmed/25462530
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272406
https://doi.org/10.1038/mtna.2014.64
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111
An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage
Fine, Eli J.; Cradick, Thomas J.; Zhao, Charles L.. - : Oxford University Press, 2014
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112
Philological analysis of Old Mongolian: consonant-vowel harmony was not universally enforced ...
Hornstein, Eli D.. - : The University of North Carolina at Chapel Hill University Libraries, 2014
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113
Tongue papillae morphology of brown-throated sloth Bradypus variegatus (SCHINZ, 1825)
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114
Formação de leitores surdos e a educação inclusiva
Martins, Sandra Eli Sartoreto de Oliveira. - : Universidade Estadual Paulista (UNESP), 2014
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115
Towards a New Model of Narrative Transformation: From the American Myth to the Green Myth
In: Communication theory. - Cary, NC : Oxford University Press 23 (2013) 1, 67-90
OLC Linguistik
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116
The use of the Internet in collecting CDI data – an example from Norway*
In: Journal of child language. - Cambridge [u.a.] : Cambridge Univ. Press 40 (2013) 3, 567-585
OLC Linguistik
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117
The story of analytic philosophy : plot and heroes
Skorupski, John; Putnam, Ruth Anna; Bar-Elli, Gilead. - London [u.a.] : Routledge, 2013
BLLDB
UB Frankfurt Linguistik
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118
A pathological core of authenticity : rereading the case of Binjamin Wilkomirski's "Bruchstücke"
In: Forum for modern language studies. - Oxford : Oxford Univ. Press 49 (2013) 1, 79-98
OLC Linguistik
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119
Infrastructure for data-intensive science : a bottom-up approach
In: Data Intensive Science (Boca Raton, 2013), p. 185-214
MPI für Psycholinguistik
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120
Coordinating the chain of utterances: an analysis of communicative flow and turn taking in an interpreted group dialogue for deaf-blind persons
In: Sign language studies. - Washington, DC : Gallaudet Univ. Press 13 (2013) 3, 350-371
BLLDB
OLC Linguistik
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