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Drosophila as a Model of Unconventional Translation in Spinocerebellar Ataxia Type 3
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In: Cells; Volume 11; Issue 7; Pages: 1223 (2022)
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Drosophila as a Model of Unconventional Translation in Spinocerebellar Ataxia Type 3
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In: Cells (2022)
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Abstract:
RNA toxicity contributes to diseases caused by anomalous nucleotide repeat expansions. Recent work demonstrated RNA-based toxicity from repeat-associated, non-AUG-initiated translation (RAN translation). RAN translation occurs around long nucleotide repeats that form hairpin loops, allowing for translation initiation in the absence of a start codon that results in potentially toxic, poly-amino acid repeat-containing proteins. Discovered in Spinocerebellar Ataxia Type (SCA) 8, RAN translation has been documented in several repeat-expansion diseases, including in the CAG repeat-dependent polyglutamine (polyQ) disorders. The ATXN3 gene, which causes SCA3, also known as Machado–Joseph Disease (MJD), contains a CAG repeat that is expanded in disease. ATXN3 mRNA possesses features linked to RAN translation. In this paper, we examined the potential contribution of RAN translation to SCA3/MJD in Drosophila by using isogenic lines that contain homomeric or interrupted CAG repeats. We did not observe unconventional translation in fly neurons or glia. However, our investigations indicate differential toxicity from ATXN3 protein-encoding mRNA that contains pure versus interrupted CAG repeats. Additional work suggests that this difference may be due in part to toxicity from homomeric CAG mRNA. We conclude that Drosophila is not suitable to model RAN translation for SCA3/MJD, but offers clues into the potential pathogenesis stemming from CAG repeat-containing mRNA in this disorder.
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URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8997593/
https://doi.org/10.3390/cells11071223
http://www.ncbi.nlm.nih.gov/pubmed/35406787
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國小英語村實施臺灣在地文化課程之研究-以高雄市K國小英語村為例 ; A Study of Taiwan Local Culture Curriculum Implementation in K Elementary English Village
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國民小學攜手計畫課後扶助注音符號補助教學之行動研究 ; Action Research in Mandarin Phonetics Symbols Remedial Teaching in the “After School Alternative Program”
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Mobilising action through management email texts: the negotiation of evaluative stance through choices in discourse and grammar ...
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Mobilising action through management email texts: the negotiation of evaluative stance through choices in discourse and grammar
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The role of form -focused communication activities in complex grammar learning: The case of relative clauses in Chinese
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In: Dissertations available from ProQuest (2005)
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