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Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
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| 2 |
Cryopreservation of swine ovarian tissue : effect of different cryoprotectants on the structural preservation of preantral follicle oocytes
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| 3 |
Effect of different cryoprotectants on the structural preservation of follicles in frozen zebu bovine (Bos indicus) ovarian tissue
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| 4 |
Morphological and ultrastructural analysis of sheep primordial follicles preserved in 0.9% saline solution and TCM 199
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| 5 |
Cryopreservation of caprine ovarian tissue using glycerol and ethylene glycol
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| 6 |
Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles
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| 7 |
Zebu (Bos indicus) ovarian preantral follicles : morphological characterization and development of an efficient isolation method
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| 8 |
Morphological and ultrastructural changes occurring during degeneration of goat preantral follicles preserved in vitro
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| 9 |
Effect of Braun-Collins and Saline solutions at different temperatures and incubation times on the quality of goat preantral follicles preserved in situ
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| 10 |
Light microscopical and ultrastructural characterization of goat preantral follicles
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| 11 |
Effect of coconut water and Braun-Collins solutions at different temperatures and incubation times on the morphology of goat preantral follicles preserved in vitro
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Abstract:
Preservation of preantral follicles becomes very important to ensure follicle quality at the onset of cryopreservation or in vitro culture. However, for domestic animals, the ovarian donor of preantral follicles for in vitro studies is commonly encountered far away from reproduction laboratories. We investigated the effectiveness of coconut water and Braun- Collins solutions on the preservation of goat preantral follicles. At the slaughterhouse, the ovarian pair of each animal was divided into 19 fragments. One ovarian fragment was immediately fixed (Control — Time 0). The other 18 fragments were randomly distributed into tubes containing 2 mL of coconut water or Braun-Collins solution at 4 °, 20 ° or 39 °C and then stored for 4, 12 or 24 h. Histological analysis showed that the storage of ovarian fragments in coconut water and Braun-Collins solutions at 20 ° or 39 °C for 12 or 24 h significantly reduced (P < 0.05) the percentage of morphologically normal preantral follicles when compared with the control. However, storage in coconut water at 20 °C for 4 h and in both solutions at 4 °C kept the percentage at control values. Ultrastructural analysis of follicles exposed to the stated conditions confirmed the integrity of preantral follicles stored at 4 °C in Braun-Collins and coconut water solutions for up to 12 and 24 h, respectively. Reduced cellular metabolism at 4 °C may explain why the best preservation of preantral follicles was at 4 °C, which may suggest a useful method for ovary transport in the future.
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Keyword:
Cabras; Folículos pré-antrais; Morfologia (Animais)
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URL: http://repositorio.unb.br/handle/10482/22634
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| 12 |
Effect of the interval of serial sections of ovarian tissue in the tissue chopper on the number of isolated caprine preantral follicles
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