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1
Frequency of Acute and Late Reaction of Non-Ionic Iodinated Contrast Media in Contrast Enhanced Computed Tomography
In: Journal of Health, Medicine and Nursing; Vol 88 (2021); 30-35 (2021)
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2
Strategies Adopted by Library Science Research Scholars to Cope with Challenges in Dissertation-Writing
In: Library Philosophy and Practice (e-journal) (2021)
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3
Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome Editing
In: Cells (2020)
Abstract: Gene editing that makes target gene modification in the genome by deletion or addition has revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 emerged as a substantial tool due to its simplicity in use, less cost and extraordinary efficiency than the conventional gene-editing tools, including zinc finger nucleases (ZFNs) and Transcription activator-like effector nucleases (TALENs). However, potential off-target activities are crucial shortcomings in the CRISPR system. Numerous types of approaches have been developed to reduce off-target effects. Here, we review several latest approaches to reduce the off-target effects, including biased or unbiased off-target detection, cytosine or adenine base editors, prime editing, dCas9, Cas9 paired nickase, ribonucleoprotein (RNP) delivery and truncated gRNAs. This review article provides extensive information to cautiously interpret off-target effects to assist the basic and clinical applications in biomedicine.
Keyword: Review
URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7407193/
http://www.ncbi.nlm.nih.gov/pubmed/32630835
https://doi.org/10.3390/cells9071608
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4
Extracting Data From Line Charts in Scanned Medical Documents
Silva de Azevedo, Kathleen. - : Auckland University of Technology, 2019
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5
Hepatocellular carcinoma (HCC) vs. non-HCC: accuracy and reliability of Liver Imaging Reporting and Data System v2018
In: Abdom Radiol (NY) (2019)
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6
Reusing Past Replies to Respond to New Email: A Case-based Reasoning Approach
Linggawa, I Wayan Sathya. - : Auckland University of Technology, 2017
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