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Contaminations contaminate common databases
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Bensch, S; Inumaru, M; Sato, Y; Lee Cruz, L; Cunningham, AA; Goodman, SJ; Levin, II; Parker, PG; Casanueva, P; Hernández, M; Moreno‐Rueda, G; Rojo, M. - : Wiley, 2020
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Abstract:
The polymerase chain reaction (PCR) is a very powerful method to detect and identify pathogens. The high sensitivity of the method however comes with a cost; any of the millions of artificial DNA copies generated by PCR can serve as a template in a following experiment. If not identified as contaminations, these may result in erroneous conclusions on the occurrence of the pathogen, thereby inflating estimates of host range and geographic distribution. In the present paper, we evaluate whether several published records of avian haemosporidian parasites, in either unusual host species or geographical regions, might stem from PCR contaminations rather than novel biological findings. The detailed descriptions of these cases are shedding light upon the steps in the work process that might lead to PCR contaminations. By increasing the awareness of this problem, it will aid in developing procedures that keep these to a minimum. The examples in the present paper are from haemosporidians of birds, however the problem of contaminations and suggested actions should apply generally to all kinds of PCR‐based identifications, not just of parasites and pathogens.
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URL: http://eprints.whiterose.ac.uk/167018/
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TEACHING AND RESEARCHING EDGAR A. POE: ; A TETRALOGY ON EXHIBIT
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Does continuous assessment in higher education support student learning?
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Collaborative Learning: Increasing Students' Engagement Outside the Classroom
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Increasing Student Engagement through Collaborative Learning Outside the Classroom
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Un cambio en tiempo real: la atenuación entre hablantes universitarios de Las Palmas de Gran Canaria
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Analysis of the genetic diversity and structure of the Spanish apple genetic resources suggests the existence of an Iberian genepool
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Escaping capture: bilingualism modulates distraction from working memory.
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In: Symplectic Elements at Oxford ; Europe PubMed Central ; PubMed (http://www.ncbi.nlm.nih.gov/pubmed/) ; Web of Science (Lite) (http://apps.webofknowledge.com/summary.do) ; Scopus (http://www.scopus.com/home.url) ; CrossRef (2012)
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Bilingualism tunes the anterior cingulate cortex for conflict monitoring
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In: Cerebral Cortex , 22 (9) 2076 - 2086. (2012) (2012)
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Abecedario: Zapoteco de Amatlán ; Alphabet Book: Amatlan Zapotec
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