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Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
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Abstract:
The objective of this study was to characterize the morphometry and ultrastructure of porcine preantral and antral follicles, especially the lipid component evolution. Ovarian tissue was processed for light microscopy. Ovarian tissue and dissected antral follicles (< 2, 2–4, and 4–6 mm) were also processed for transmission electron microscopy using routine methods and using an osmium-imidazole method for lipid detection. Primordial follicles (34 ± 5 μm in diameter, mean ± SD) had one layer of flattened-cuboidal granulosa cells around the oocyte, primary follicles (40 ± 7 μm) had a single layer of cuboidal granulosa cells around the oocyte, and secondary follicles (102 ± 58 μm) had two or more layers of cuboidal granulosa cells around the oocyte. Preantral follicle oocytes had many round mitochondria and both rough and smooth endoplasmic reticulum. In oocytes of primordial and primary follicles, lipid droplets were abundant and were mostly located at the cell poles. In secondary and antral follicles, the zona pellucida completely surrounded the oocyte, whereas some microvilli and granulosa cells projected through it. Numerous electron-lucent vesicles and vacuoles were present in the oolemma of secondary and antral follicles. Based on osmium-imidazole staining, most of these structures were shown to be lipid droplets. As the follicle developed, the appearance of the lipid droplets changed from small and black to large and gray, dark or dark with light streaks, suggesting that their nature may change over time. In summary, although porcine follicles and oocytes had many similarities to those of other mammalian species, they were rich in lipids, with lipid droplets with varying morphological patterns as the follicle developed.
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Keyword:
Folículos pré-antrais; Lipídios; Suínos
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URL: http://repositorio.unb.br/handle/10482/17621
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| 2 |
Cryopreservation of swine ovarian tissue : effect of different cryoprotectants on the structural preservation of preantral follicle oocytes
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Effect of different cryoprotectants on the structural preservation of follicles in frozen zebu bovine (Bos indicus) ovarian tissue
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Morphological and ultrastructural analysis of sheep primordial follicles preserved in 0.9% saline solution and TCM 199
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Cryopreservation of caprine ovarian tissue using glycerol and ethylene glycol
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| 6 |
Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles
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Zebu (Bos indicus) ovarian preantral follicles : morphological characterization and development of an efficient isolation method
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| 8 |
Morphological and ultrastructural changes occurring during degeneration of goat preantral follicles preserved in vitro
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| 9 |
Effect of Braun-Collins and Saline solutions at different temperatures and incubation times on the quality of goat preantral follicles preserved in situ
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Light microscopical and ultrastructural characterization of goat preantral follicles
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Effect of coconut water and Braun-Collins solutions at different temperatures and incubation times on the morphology of goat preantral follicles preserved in vitro
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| 12 |
Effect of the interval of serial sections of ovarian tissue in the tissue chopper on the number of isolated caprine preantral follicles
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