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Ultrastructural characterization of porcine oocytes and adjacent follicular cells during follicle development : lipid component evolution
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| 2 |
Cryopreservation of swine ovarian tissue : effect of different cryoprotectants on the structural preservation of preantral follicle oocytes
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| 3 |
Effect of different cryoprotectants on the structural preservation of follicles in frozen zebu bovine (Bos indicus) ovarian tissue
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| 4 |
Morphological and ultrastructural analysis of sheep primordial follicles preserved in 0.9% saline solution and TCM 199
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Cryopreservation of caprine ovarian tissue using glycerol and ethylene glycol
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| 6 |
Effects of lowered temperatures and media on short-term preservation of zebu (Bos indicus) preantral ovarian follicles
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| 7 |
Zebu (Bos indicus) ovarian preantral follicles : morphological characterization and development of an efficient isolation method
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Abstract:
Preantral follicles are a major source of oocytes, and their utilization as an important tool to store large number of female gametes for future use in reproductive programs has been investigated. The increasing importance of studies in this subject, together with the important role of Zebu cattle in the economy of tropical and subtropical countries as well as their well-known differences from European cattle, led to this research. The present study aims to determine the best size interval for sectioning ovarian tissue to isolate preantral follicles from Zebu cows using a tissue chopper and to evaluate the follicular quality after isolation. Furthermore, it aims to provide information about the Zebu cow preantral follicle population and use this data (as a control) to evaluate the effectiveness of the tested isolation method. Testing eight different tissue sectioning size intervals, it was possible to conclude that the 125-pm-section interval is shown to be better than the intervals of 25, 50, 175 and 200 pm to isolate preantral follicles from Zebu cow ovaries. The 125- pm interval allowed the recovery of 26,050±1611 (meaniS.E.M.) preantral follicles per one-half ovary, while the number of preantral follicles in situ estimated by evaluation of histological sections was 35,28812342 per one-half ovary. Thus, the mean (iS.E.M.) recovery rate (=[number of preantral follicles isolated/number of preantral follicles in situ in the same ovary] x 100) was 74.3±4.3%. The morphometrical analysis showed that Bos indicus preantral follicles are similar to B. taurus preantral follicles based on previous reports. In conclusion, this study showed that a simple, mechanical method can be used effectively to isolate a large number of intact preantral follicles from Zebu cow ovaries, with a high recovery rate.
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Keyword:
Folículos - qualidade; Folículos pré-antrais; Zebu
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URL: http://repositorio.unb.br/handle/10482/19324
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| 8 |
Morphological and ultrastructural changes occurring during degeneration of goat preantral follicles preserved in vitro
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| 9 |
Effect of Braun-Collins and Saline solutions at different temperatures and incubation times on the quality of goat preantral follicles preserved in situ
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| 10 |
Light microscopical and ultrastructural characterization of goat preantral follicles
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| 11 |
Effect of coconut water and Braun-Collins solutions at different temperatures and incubation times on the morphology of goat preantral follicles preserved in vitro
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| 12 |
Effect of the interval of serial sections of ovarian tissue in the tissue chopper on the number of isolated caprine preantral follicles
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